Difference between revisions of "Part:BBa K2595005"

Latest revision as of 14:21, 17 October 2018

RyhB

RyhB is a 90 nucleotides-long, non-coding RNA with AU-rich regions. RyhB RNA down-regulates the quantity of iron-using proteins when there is limitation on iron hence regulating a set of iron-storage. The structure of the RyhB RNA is critical for its function and regulation. In E.Coli, the AU-rich region of RyhB binds to Hfq, the RNA chaperone, to target mRNA sodB and result in changes in mRNA transcription, translation or stability. RyhB binding to the Hfq protein is essential in increasing its interaction with its target messages.

Usage and Biology

This synthetic version of RyhB follows the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RyhB can regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-This synthetic version of RyhB follows the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RyhB can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
+This synthetic version of RyhB follows the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RyhB can regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.
 <span class='h3bb'>Sequence and Features</span>