Difference between revisions of "Part:BBa K2595007"

Latest revision as of 14:24, 17 October 2018

RprA

RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) (Majdalani et al., 2001). Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA (Soper et al., 2010).

RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation (Mika et al., 2012).

Usage and Biology

This synthetic version of RprA follow the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2595007 short</partinfo>
-RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) [1]. Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA  [3].
+RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) (Majdalani et al., 2001). Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA  (Soper et al., 2010).
-RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation [5].
+RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation (Mika et al., 2012).
 ===Usage and Biology===
-This synthetic version of RprA follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
+This synthetic version of RprA follow the same principles as its wild type partner to regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can regulate the expression of the gene that contains the complementary regions for its targeting sequence.  Ligation with the desired targeting sequence does not create any scar in the sRNA sequence.
 <span class='h3bb'>Sequence and Features</span>