Difference between revisions of "Part:BBa K2719012:Design"
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For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with <i>E. coli</i> BL21 (DE3) and with pSB1C3 plasmid. (Figure 1) | For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with <i>E. coli</i> BL21 (DE3) and with pSB1C3 plasmid. (Figure 1) | ||
| − | "https://static.igem.org/mediawiki/2018/ | + | "https://static.igem.org/mediawiki/2018/d/db/T--TecCEM--BBa_K2719012Diagram.png" |
<p><i>Figure 1.</i> Diagram of BBa_K2719012, leptin expression device.</p> | <p><i>Figure 1.</i> Diagram of BBa_K2719012, leptin expression device.</p> | ||
Latest revision as of 13:27, 17 October 2018
TCD5 coding device with blue chromoprotein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1257
Design Notes
For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with E. coli BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)
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Figure 1. Diagram of BBa_K2719012, leptin expression device.
Source
TCD5 coding device with blue chromoprotein