Difference between revisions of "Part:BBa K2622026"
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<partinfo>BBa_K2622026 short</partinfo>
 
<partinfo>BBa_K2622026 short</partinfo>
  
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Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus, so that it could be displayed and detected in the outer surface of the membrane. Lpp protein does not have a signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's based on a T7 RNR polymerase as it has T7 promoter and terminator.
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Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. Lpp protein does not have a signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.
  
 
This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI site between T7 promoter and RBS that must be avoided.
 
This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI site between T7 promoter and RBS that must be avoided.

Revision as of 13:19, 17 October 2018


Lpp-OmpA-His

Lpp-OmpA-His has a 6x His-tag attached to Lpp-OmpA's (BBa_K1991004) C-terminus via glycine/serine linker, so that it could be displayed and detected in the outer surface of the membrane. Lpp protein does not have a signal sequence as this part was designed to be used in vitro systems (liposomes, nanodiscs). This part should be expressed in IVTT's or bacteria lysates based on a T7 RNR polymerase as it has T7 promoter and terminator.

This composite part has a RFC10 prefix and suffix, though it has incompatible XbaI site between T7 promoter and RBS that must be avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 39
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 456
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 39
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 39
    Illegal NgoMIV site found at 411
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20