Difference between revisions of "Part:BBa K2719006:Experience"
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===Applications of BBa_K2719006=== | ===Applications of BBa_K2719006=== | ||
| − | This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into <i>E.coli</i> DH5a. The colonies obtained can be seen on figure 1. | + | <p>This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into <i>E.coli</i> DH5a. The colonies obtained can be seen on figure 1.</p> |
| + | [[file:T--TecCEM--Col5Colonies.png]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with Collagen V-like.</p> | ||
| − | Then the plasmid was extracted and run on an agarose gel electrophoresis, latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis. | + | |
| + | <p>Then the plasmid was extracted and run on an agarose gel electrophoresis, latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.</p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 17:01, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2719006
This part was cloned on pSB1C3 using EcoRI and PstI and latter transformed into E.coli DH5a. The colonies obtained can be seen on figure 1.
Figure 1. Colonies transformed with Collagen V-like.
Then the plasmid was extracted and run on an agarose gel electrophoresis, latter it was digested using NotI to probe the presence of the insert, the restriction was also run on a agarose gel electrophoresis.
User Reviews
UNIQcffa4512bf642000-partinfo-00000000-QINU UNIQcffa4512bf642000-partinfo-00000001-QINU