Difference between revisions of "Part:BBa K2818002"

Revision as of 16:23, 17 October 2018

Cas13b-NES-ADAR

dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required. It is an optimized construct obtained developed by Zhang Feng's lab (Feng et. al., 2017) to mediate efficient adenosine to inosine on specific positions on mRNA target, which can be programmed by a specific guide RNA. This construct is submitted as it is currently not in the iGEM registry and it acts as an important basis for comparison when we characterize our new part this year (<a href="https://parts.igem.org/Part:BBa_K2818001">BBa_K2818001</a>).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2884
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1787
Illegal BamHI site found at 839
Illegal BamHI site found at 3310
Illegal XhoI site found at 4011
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1520
Illegal AgeI site found at 2396
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2818002 short</partinfo>
-dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required.
+dPspCas13b-ADAR2DD(E488Q) is a fusion protein that catalyzes the hydrolytic deamination of adenosine to form inosine in RNA molecules when used in conjunction with a guide RNA. Although there are preferred motifs, no Protospacer Adjacent Motif (PAM) is required. It is an optimized construct obtained developed by Zhang Feng's lab (Feng et. al., 2017) to mediate efficient adenosine to inosine on specific positions on mRNA target, which can be programmed by a specific guide RNA. This construct is submitted as it is currently not in the iGEM registry and it acts as an important basis for comparison when we characterize our new part this year (<a href="https://parts.igem.org/Part:BBa_K2818001">BBa_K2818001</a>).
 <!-- Add more about the biology of this part here