Difference between revisions of "Part:BBa K2719008:Experience"
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===Applications of BBa_K2719008=== | ===Applications of BBa_K2719008=== | ||
| − | <p>To confirm the presence of Leptin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in <i>Escherichia coli</i> DH5a. (Figure | + | <p>To confirm the presence of Leptin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in <i>Escherichia coli</i> DH5a. (Figure 1).</p> |
| + | [[file:T--TecCEM--LepColonies.png|500px]] | ||
| + | <p><i>Figure 1.</i> Colonies transformed with leptin coding site. </p> | ||
<p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 4 and 3)</p> | <p>To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 4 and 3)</p> | ||
Revision as of 17:11, 17 October 2018
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Applications of BBa_K2719008
To confirm the presence of Leptin, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1).
Figure 1. Colonies transformed with leptin coding site.
To prove the presence of the plasmid inside the colonies, two agarose gels were elaborated, one for watch the entire plasmid and the second one for the plasmid with a previously restriction process using NotI, so the last one could confirm the presence of Leptin. (Figure 4 and 3)
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