Difference between revisions of "Part:BBa K2789024:Design"
 
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<partinfo>BBa_K2789024 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2789024 SequenceAndFeatures</partinfo>
  
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Primer:<br/>
 
  
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For :5’- CGGAATTCGCGGCCGCTTCTAGAG ATGAAAGCCACCAAACTGGTTCTGGG-3’<br/>
 
  
 +
===Design Notes===
 +
This part is coding sequence of OmpA and lectin. You can add promoter and terminator to make it express the lectin on the surface of bacteria to bind with agents that have a lot of negative charges.<br/>
  
−
Rev:5’- GGTACTAGTAGCGGCCGCTGCAG TTATTACGGACCCGGACTGCTACCTGTG-3’<br/>
+
We synthesized the whole pathway in Pet28a, and use PCR to make this standard coding sequence.<br/>
 +
Primer:<br/>
  
 +
For :5’- CGGAATTCGCGGCCGCTTCTAGAG ATGAAAGCCACCAAACTGGTTCTGGG-3’<br/>
  
−
===Design Notes===
 
−
This part is coding sequence of OmpA and lectin. You can add promoter and terminator to make it express the lectin on the surface of bacteria to bind with agents that have a lot of negative charges.
 
  
 +
Rev:5’- GGTACTAGTAGCGGCCGCTGCAG TTATTACGGACCCGGACTGCTACCTGTG-3’<br/>
  
  

Latest revision as of 12:48, 17 October 2018


Lectin-OmpA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 307
    Illegal AgeI site found at 496
    Illegal AgeI site found at 634
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is coding sequence of OmpA and lectin. You can add promoter and terminator to make it express the lectin on the surface of bacteria to bind with agents that have a lot of negative charges.

We synthesized the whole pathway in Pet28a, and use PCR to make this standard coding sequence.
Primer:

For :5’- CGGAATTCGCGGCCGCTTCTAGAG ATGAAAGCCACCAAACTGGTTCTGGG-3’


Rev:5’- GGTACTAGTAGCGGCCGCTGCAG TTATTACGGACCCGGACTGCTACCTGTG-3’


Source

We found it originally in genome of Green Algae.

References