Difference between revisions of "Part:BBa K2719006"

Latest revision as of 12:55, 17 October 2018

Collagen V - Like

Collagen V is one of the main proteins involved in the extracellular matrix, thus it would be important in the structure of our scaffold. The main challenge for producing recombinant collagen V is that for its correct structure it requires post-traductional modifications, specially the hydroxylation of proline. To overcome this challenge, we designed this part to incorporate hydroxyproline, a non-canonical amino acid. For its proper incorporation, this part is optimized for its use on E.coli Rosetta (DE3). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 92

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 <partinfo>BBa_K2719006 short</partinfo>
-Collagen V is one of the main proteins involved in the extracellular matrix, thus it would be important in the structure of our scaffold.  The main challenge for producing recombinant collagen V is that for its correct structure it requires post-traductional modifications, specially the hydroxylation of proline.  To overcome this challenge, we designed this part to incorporate hydroxyproline, a non-canonical amino acid.  For its proper incorporation, this part is optimized for its use on E.coli Rosetta (DE3).
+Collagen V is one of the main proteins involved in the extracellular matrix, thus it would be important in the structure of our scaffold.  The main challenge for producing recombinant collagen V is that for its correct structure it requires post-traductional modifications, specially the hydroxylation of proline.  To overcome this challenge, we designed this part to incorporate hydroxyproline, a non-canonical amino acid.  For its proper incorporation, this part is optimized for its use on <i>E.coli</i> Rosetta (DE3).
 <!-- Add more about the biology of this part here
 ===Usage and Biology===