Difference between revisions of "Part:BBa K2771030:Experience"
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<h2>USP-Brazil Characterization</h2> | <h2>USP-Brazil Characterization</h2> | ||
| − | This part has been tested alongside with six quorum sensing promoters, pLux ([https://parts.igem.org/Part:BBa_K2771021 BBa_K2771021]), pLas ([https://parts.igem.org/Part:BBa_K2771022 BBa_K2771022]), pRhl([https://parts.igem.org/Part:BBa_K2771023 BBa_K2771023]), pCin([https://parts.igem.org/Part:BBa_K2771024 BBa_K2771024]), pTra ([https://parts.igem.org/Part:BBa_K2771025 BBa_K2771025]) and pRpa ([https://parts.igem.org/Part:BBa_K2771026 BBa_K2771026]). Assay in 2mL LB in 24-well plate, shaking at 180rpm and 37ºC, 2.5 mM arabinose, taking samples every 3hrs: | + | This part has been tested alongside with six quorum sensing promoters, pLux ([https://parts.igem.org/Part:BBa_K2771021 BBa_K2771021]), pLas ([https://parts.igem.org/Part:BBa_K2771022 BBa_K2771022]), pRhl([https://parts.igem.org/Part:BBa_K2771023 BBa_K2771023]), pCin([https://parts.igem.org/Part:BBa_K2771024 BBa_K2771024]), pTra ([https://parts.igem.org/Part:BBa_K2771025 BBa_K2771025]) and pRpa ([https://parts.igem.org/Part:BBa_K2771026 BBa_K2771026]). Assay in 2mL LB in 24-well plate, shaking at 180rpm and 37ºC, 2.5 mM arabinose, taking samples every 3hrs. Note that Y axis is in ratiometric activity, because reporter is measured as a ratio of YFP/CFP fluorescence: |
[[File:T--USP-Brazil--Lux_Las_assay.png|500px|thumb|none|alt=experiment 1.]] | [[File:T--USP-Brazil--Lux_Las_assay.png|500px|thumb|none|alt=experiment 1.]] | ||
[[File:T--USP-Brazil--Others assay.png|500px|thumb|none|alt=experiment 2.|Figures 1 and 2: Labels refer as Sender plasmid:Receiver plasmid, combining BBa_K2771030 with different quorum sensing promoters]] | [[File:T--USP-Brazil--Others assay.png|500px|thumb|none|alt=experiment 2.|Figures 1 and 2: Labels refer as Sender plasmid:Receiver plasmid, combining BBa_K2771030 with different quorum sensing promoters]] | ||
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| + | We also did an assay comparing activity when induced by arabinose and when induced directly by HSL. Auto-inducing showed slower response, but much higher threshold: | ||
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| + | [[File:T--USP-Brazil--ara HSL assay.png|500px|thumb|none|alt=experiment 3.]] | ||
===Applications of BBa_K2771030=== | ===Applications of BBa_K2771030=== | ||
Revision as of 11:51, 17 October 2018
USP-Brazil Characterization
This part has been tested alongside with six quorum sensing promoters, pLux (BBa_K2771021), pLas (BBa_K2771022), pRhl(BBa_K2771023), pCin(BBa_K2771024), pTra (BBa_K2771025) and pRpa (BBa_K2771026). Assay in 2mL LB in 24-well plate, shaking at 180rpm and 37ºC, 2.5 mM arabinose, taking samples every 3hrs. Note that Y axis is in ratiometric activity, because reporter is measured as a ratio of YFP/CFP fluorescence:
We also did an assay comparing activity when induced by arabinose and when induced directly by HSL. Auto-inducing showed slower response, but much higher threshold:
Applications of BBa_K2771030
User Reviews
UNIQf30825db22f90b5b-partinfo-00000000-QINU UNIQf30825db22f90b5b-partinfo-00000001-QINU