Difference between revisions of "Part:BBa K2719000:Experience"
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To confirm the presence of GST, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1) | To confirm the presence of GST, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1) | ||
| − | [[file:T--TecCEM--GSTColonies.png]] | + | [[file:T--TecCEM--GSTColonies.png|500PX]] |
<p>Figure 1. Transformed GST Colonies</p> | <p>Figure 1. Transformed GST Colonies</p> | ||
Revision as of 12:08, 17 October 2018
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Applications of BBa_K2719000
To confirm the presence of GST, it was cloned in pSB1C3 using EcoRI and PstI for the restriction and T4 ligase for the ligation. After that, it was transformed in Escherichia coli DH5a. (Figure 1)
Figure 1. Transformed GST Colonies
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