Difference between revisions of "Part:BBa K2686006:Experience"
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| − | The part was designed and constructed in pET14 vector | + | The part was designed and constructed in the original pET14 vector from <bbpart>K2686001</bbpart>. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). |
| + | The HexaHistidine-OT1 Encapsulin construct was also expressed and presented to dendritic cells to check its ability to induce the presentation of the OT1 antigen on MHC I complexes on the dendritic cells. | ||
| + | |||
Revision as of 12:47, 17 October 2018
The part was designed and constructed in the original pET14 vector from K2686001. The sequence was confirmed using Sanger sequencing. The part was expressed in the pET14 vector using a BL21 DE3 TX-TL (Sun et al., 2013) cell free expression system. After expression and purification, the part was validated in multiple ways. First, multiple SDS-PAGE experiments indicate that our expressed protein is at the correct hypothetical size, which was further confirmed by lysine BODIPY tRNA expression (32.96kDa for the monomer and 1.98MDa for the 60-mer). The HexaHistidine-OT1 Encapsulin construct was also expressed and presented to dendritic cells to check its ability to induce the presentation of the OT1 antigen on MHC I complexes on the dendritic cells.
Applications of BBa_K2686006
User Reviews
UNIQ7811759f0e817e5b-partinfo-00000001-QINU UNIQ7811759f0e817e5b-partinfo-00000002-QINU