Difference between revisions of "Part:BBa K2569031:Design"
Line 9: Line 9:
  
 
===Source===
 
===Source===
−
NicA2 comes from the genome of Pseudomonas Putita s16. INSDC: CP002870.1. And the constitutive promoter comes from our promoter library.  
+
NicA2 comes from the genome of Pseudomonas Putita s16. INSDC: CP002870.1. And the constitutive promoter comes from our promoter library.  
  
 
===References===
 
===References===

Revision as of 10:51, 17 October 2018

PdnaA -NicA2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nicotine oxidase NicA2 behind our strongest constitutive promoter leading to enhance nicotine degradationin Pseudomonadaceae compared to original nicA2 gene.

Source

NicA2 comes from the genome of Pseudomonas Putita s16. INSDC: CP002870.1. And the constitutive promoter comes from our promoter library.

References