Difference between revisions of "Part:BBa J364000:Experience"
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<b>SCU-China 2018:</b> | <b>SCU-China 2018:</b> | ||
| − | <p><b>Experiment conditions:</b> | + | <p><b>Experiment conditions:</b></p> |
| − | <p>1. 37℃ incubator for bacteria; | + | <p>1. 37℃ incubator for bacteria; </p> |
| − | <p>2.LB liquid medium. | + | <p>2.LB liquid medium.</p> |
| − | <p>We use this part (BBa_J364000) as our original source of GFP proteins. As our expectation, this part can work normally under our experiment conditions. | + | <p>We use this part (BBa_J364000) as our original source of GFP proteins. As our expectation, this part can work normally under our experiment conditions.</p> |
| − | <p>For the modification, we added a spacer sequence and NGG site just on the upstream of the promoter for repression of GFP expression. And this modification is very successful because the supression is obvious according to the Figure 1 below. | + | <p>For the modification, we added a spacer sequence and NGG site just on the upstream of the promoter for repression of GFP expression. And this modification is very successful because the supression is obvious according to the Figure 1 below.</p> |
[[File:SCU_China-2018_dCas9_repression.png]] | [[File:SCU_China-2018_dCas9_repression.png]] | ||
| − | <p>As the picture shows, the part:BBa_J364000 and our part: BBa_K2611001 can both function well. And for the <b>improvement:</b> this part's expression can be regulated under the control of dCas9 system. | + | <p>As the picture shows, the part:BBa_J364000 and our part: BBa_K2611001 can both function well. And for the <b>improvement:</b> this part's expression can be regulated under the control of dCas9 system.</p> |
=== 2017 Georgia State iGEM Team === | === 2017 Georgia State iGEM Team === | ||
Revision as of 08:44, 17 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J364000
User Reviews
UNIQe77a4b63b40723e1-partinfo-00000000-QINU UNIQe77a4b63b40723e1-partinfo-00000001-QINU
SCU-China 2018:
Experiment conditions:
1. 37℃ incubator for bacteria;
2.LB liquid medium.
We use this part (BBa_J364000) as our original source of GFP proteins. As our expectation, this part can work normally under our experiment conditions.
For the modification, we added a spacer sequence and NGG site just on the upstream of the promoter for repression of GFP expression. And this modification is very successful because the supression is obvious according to the Figure 1 below.
As the picture shows, the part:BBa_J364000 and our part: BBa_K2611001 can both function well. And for the improvement: this part's expression can be regulated under the control of dCas9 system.
2017 Georgia State iGEM Team
Georgia State Growth Medium Study
