Difference between revisions of "Part:BBa K2542008"

(→‎Experiment)
Line 12: Line 12:
 
<partinfo>BBa_K2542008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2542008 SequenceAndFeatures</partinfo>
 
===Experiment===
 
===Experiment===
−
We placed the homology arms (BBa_K2542008 & BBa_K2542007) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.  
+
We placed the homology arms ([https://parts.igem.org/Part:BBa_K2542007 BBa_K2542007] & [https://parts.igem.org/Part:BBa_K2542008 BBa_K2542008]) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.  
 
[[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|350px]]
 
[[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|350px]]
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 08:27, 17 October 2018


Part of the transposable element of Saccharomyces cerevisiae.

It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Experiment

We placed the homology arms (BBa_K2542007 & BBa_K2542008) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.

T--NEFU China--homologous recombination PCR.jpg