Difference between revisions of "Part:BBa K2542008"
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<partinfo>BBa_K2542008 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2542008 SequenceAndFeatures</partinfo> | ||
===Experiment=== | ===Experiment=== | ||
| − | We placed the homology arms at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful. | + | We placed the homology arms (BBa_K2542008 & BBa_K2542007) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful. |
[[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|350px]] | [[Image:T--NEFU_China--homologous_recombination_PCR.jpg|thumb|350px]] | ||
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Revision as of 08:25, 17 October 2018
Part of the transposable element of Saccharomyces cerevisiae.
It' a part of the transposable element of Saccharomyces cerevisiae, which can be used to perform homologous recombination of DNA to insert the sequence of interest into the genome of Saccharomyces cerevisiae. The other is BBa_K2542007.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experiment
We placed the homology arms (BBa_K2542008 & BBa_K2542007) at both ends of the insertion sequence.Then we extracted the yeast genome and used PCR to test whether the homologous recombination was successful.
