Difference between revisions of "Part:BBa K2611004"
 
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<partinfo>BBa_K2611004 short</partinfo>
 
<partinfo>BBa_K2611004 short</partinfo>
  
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We designed this sgRNA that are complementary to the J23100 constitutive promoter using a special algorithm. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.  
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We designed this sgRNA that are complementary to the J23100 constitutive promoter by BLAST all spacer sequence generated by GFP open reading frame with the genome of E. coli BL21 strain. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 07:33, 17 October 2018


sgRNA (J23100)

We designed this sgRNA that are complementary to the J23100 constitutive promoter by BLAST all spacer sequence generated by GFP open reading frame with the genome of E. coli BL21 strain. This sgRNA can direct dCas9 protein to combine with the J23100 constitutive promoter to block the transcription. As a result, the expression of the downstream will decrease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]