Difference between revisions of "Part:BBa K2623013:Experience"

(→‎Identification)
(→‎Identification)
Line 2: Line 2:
 
====Identification====
 
====Identification====
 
We do Enzyme-Cut identification to certify the plasmid is correct. We use the EcoR I and Pst I to cut the plasmid, which is the pSB1C3 containg the DNA sequence of BBa K2623013.(Fig.1)<br>
 
We do Enzyme-Cut identification to certify the plasmid is correct. We use the EcoR I and Pst I to cut the plasmid, which is the pSB1C3 containg the DNA sequence of BBa K2623013.(Fig.1)<br>
−
[[Image:K2623013.png|thumb|200px|Fig.1]]
+
<table><tr><th>[[Image:K2623013.png|thumb|200px|Fig.1 The result of enzyme indentification]]</th><th></table>
−
<br>
+
 
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
−
<br>
+
 
<br>
 
<br>
 
<br>In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.<br>
 
<br>In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.<br>

Revision as of 18:36, 17 October 2018

Applications of BBa_K2623013

Identification

We do Enzyme-Cut identification to certify the plasmid is correct. We use the EcoR I and Pst I to cut the plasmid, which is the pSB1C3 containg the DNA sequence of BBa K2623013.(Fig.1)

Fig.1 The result of enzyme indentification



In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.
Since the Kai system is designed for periodic output of signals in Escherichia coli, we evaluateD the changes of fluorescent signals over time. Here are the curves we made. More details can be found in http://2018.igem.org/Team:XMU-China/Measurement

Fig.3 The bioluminescence at different times in GroupA. The negative value is cause by the value of the control group.
Fig.4 The bioluminescence at different times in GroupB. The negative value is cause by the value of the control group.

Fig.5 The fitting curve of the oscillator in GroupA
Fig.6 The fitting curve of the oscillator in GroupB


More details can be seen in http://2018.igem.org/Team:XMU-China/Results

User Reviews

UNIQa85fab5935d60564-partinfo-00000000-QINU UNIQa85fab5935d60564-partinfo-00000001-QINU