Difference between revisions of "Part:BBa K2832005"

Revision as of 22:45, 17 October 2018

RpoE Inducible mRFP1 Generator (RpoE --> bamEp --> mRFP1)

General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it allows for the expression of RFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 13
Illegal AgeI site found at 769
Illegal AgeI site found at 881
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2832005 short</partinfo>
-General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon.
+General stress response sigma factor. Responds to stress on membrane and periplasmic proteins. Binding of unassembled outer membrane proteins to the protease DegS stimulate its cleavage of the regulatory protein RseA, which tethers sigma E to the inner membrane. bamE is a member of the sigma E regulon. This potential sound sensitive promoter is attached to the sequence that codes for RFP. When the promoter is activated it allows for the expression of RFP.
 <!-- Add more about the biology of this part here