Difference between revisions of "Part:BBa K2560075:Design"
 
Line 18: Line 18:
  
 
<b> Forward Oligo:</b>
 
<b> Forward Oligo:</b>
−
CTCGCGCTTACTGGAGACGAGCT
+
CTCGAACACGTCTCGAACAGGAG
  
 
<b> Reverse Oligo:</b>
 
<b> Reverse Oligo:</b>
−
CTCAAGCTCGTCTCCAGTAAGCG
+
CTCACTCCTGTTCGAGACGTGTT

Latest revision as of 00:18, 17 October 2018


Phytobrick version of 5'Con1_L1 Short Res Connector


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This connectors were designed to enable flexible cloning of LVL 2 plasmids. For more informations feel free to visit our Design Page.

Source

The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.

Forward Oligo: CTCGAACACGTCTCGAACAGGAG

Reverse Oligo: CTCACTCCTGTTCGAGACGTGTT