Difference between revisions of "Part:BBa K108014:Experience"

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We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter. Therefore we have used the new phaR and promoter sequence as our new template. Due to the excessive GC in the sequence, we have it codon optimised to E.coli and generated the part through the IDT company service.
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We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter (BBa_k2739002). Therefore we have used the new promoter sequence as our new template and generated the part through the IDT company service.
  
 
Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.
 
Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.

Latest revision as of 01:21, 18 October 2018


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Applications of BBa_K108014

User Reviews

UNIQfa23503b6e8d2514-partinfo-00000000-QINU UNIQfa23503b6e8d2514-partinfo-00000001-QINU

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Edinburgh OG 2018

We access the online NCBI data (NCBI: AM260479.1) and use reference 1 as guideline and found out the current available the functional promoter is being 134bp shorter (BBa_k2739002). Therefore we have used the new promoter sequence as our new template and generated the part through the IDT company service.

Reference 1. Yamada M, Takahashi S, Okahata Y, Doi Y, Numata K. (2013 ) Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]. AMB Express. 3(1):6.

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