Difference between revisions of "Part:BBa K2812003:Design"
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The designed sequence was synthesized ''de novo by'' IDT. | The designed sequence was synthesized ''de novo by'' IDT. | ||
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===References=== | ===References=== |
Revision as of 21:53, 16 October 2018
Coding sequence for trunctated Lysostaphin regulated by T7-promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 164
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial starting sequence for truncated lysostaphin was taken from BBa_K748002. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli.
Source The designed sequence was synthesized de novo by IDT.