Difference between revisions of "Part:BBa K2560070:Design"
(→Design Notes) |
(→Source) |
||
Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
− | + | <html> | |
+ | |||
+ | The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector <a href="https://parts.igem.org/Part:BBa_K2560002">BBa_K2560002</a> using Golden Gate assembly. If you stuggle with <i> de novo </i> synthesis we recomended this | ||
+ | <a href="https://parts.igem.org/Help:Promoters/Construction">site</a>. | ||
+ | |||
+ | </html> | ||
+ | |||
+ | <b> Forward Oligo:</b> | ||
+ | CTCGCGCTTACTGGAGACGAGCT | ||
+ | |||
+ | <b> Reverse Oligo:</b> | ||
+ | CTCAAGCTCGTCTCCAGTAAGCG | ||
===References=== | ===References=== |
Revision as of 23:46, 16 October 2018
Phytobrick version of 3'Con1_L1 Connector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
The part was created by annealing single stranded oligonucleotides and subsequent integration into the part entry vector BBa_K2560002 using Golden Gate assembly. If you stuggle with de novo synthesis we recomended this site.
Forward Oligo: CTCGCGCTTACTGGAGACGAGCT
Reverse Oligo: CTCAAGCTCGTCTCCAGTAAGCG