Difference between revisions of "Part:BBa K209496"

Revision as of 20:53, 16 October 2018

AarI B-D part, FRB

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

2018 Peking Team improvement

Team Peking 2018 improved this part.

Firstly, we fused this this part with yEGFP, which made it visible in the yeast through fluorescent microscope.

https://parts.igem.org/Part:BBa_K2601007

Then we characterized the expression of Frb-yEGFP with 2 promoters pTet07 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601021; https://parts.igem.org/Part:BBa_K2601023

Moreover, we fused Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.

https://parts.igem.org/Part:BBa_K2601010

Finally, we characterized the expression of Frb-yEGFP-HOTag6 with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.

https://parts.igem.org/Part:BBa_K2601032 https://parts.igem.org/Part:BBa_K2601033 https://parts.igem.org/Part:BBa_K2601034

@@ Line 23: / Line 23: @@
 Team Peking 2018 improved this part.
-First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
+Firstly, we fused this this part with yEGFP, which made it visible in the yeast through fluorescent microscope.
 https://parts.igem.org/Part:BBa_K2601007
-Then, we drive the expression of Frb-yEGFP with 3 promoters pTet07 and PDH3. The expression of these promoters were measure by flow cytometry.
+Then we characterized the expression of Frb-yEGFP with 2 promoters pTet07 and PDH3. The expression of these promoters was measured through flow cytometry.
-https://parts.igem.org/Part:BBa_K2601021
+https://parts.igem.org/Part:BBa_K2601021;
 https://parts.igem.org/Part:BBa_K2601023
-What's more, we fuse Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.
+Moreover, we fused Frb-yEGFP with HOtag6. This part can form phase separation with FKBP-yEGFP-HOTag3 in the presence of rapamycin.
 https://parts.igem.org/Part:BBa_K2601010
-Then, we drive the expression of Frb-yEGFP-HOTag6 with 2 promoters pTet07, pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
+Finally, we characterized the expression of Frb-yEGFP-HOTag6 with 3 promoters pTet07, pTEF1 and PDH3. The expression of these promoters was measured through flow cytometry.
 https://parts.igem.org/Part:BBa_K2601032
 https://parts.igem.org/Part:BBa_K2601033
 https://parts.igem.org/Part:BBa_K2601034