Difference between revisions of "Part:BBa K2656106"
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Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1). | Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1). | ||
| − | [[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Assembled | + | [[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Assembled transcriptional unit. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator]] |
Revision as of 19:50, 16 October 2018
GFP transcriptional unit 2
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
It is a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green color with medium intensity.
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
- BBa_K2656005: the J23102 constitutive promoter in its Golden Braid compatible version
- BBa_K2656009: the J61100 strong ribosome biding site in its Golden Braid compatible version
- BBa_K2656022: the BBa_K2656022 GFPmut3b sequence in its Golden Braid standardized version
- Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version
It has been used to characterize the constitutive BBa_K2656005 promoter. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so the results of the next graph have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
| Table 1. Optimized parameters for the constitutive model with TU BBa_K2656106. | |||
| Parameter | Value | ||
| Constitutive transcription rate CR | CR = 539.5 min-1 | ||
| Dilution rate μ | μ = 0.0058 min-1 | ||
| PoI degradation rate | Promoter Experiment: dp = | ||
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]