Difference between revisions of "Part:BBa K209023"
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==Result==
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===2018 Peking Team improvement===
 +
Team Peking 2018 improved this part.
 +
 
 +
First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope.
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601008</a><br/>
 +
 
 +
Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
 +
 
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025</a><br/>
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026</a><br/>
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027</a><br/>
 +
 
 +
What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin.
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a><br/>
 +
 
 +
Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a><br/>
 +
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a><br/>

Revision as of 19:34, 16 October 2018


Aar1 C-D part, FKBP

dimerizes with FRB when rapamycin is present

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2018 Peking Team improvement

Team Peking 2018 improved this part.

First, we fuse this this part with yEGFP, which make it visible in the yeast under fluorescent microscope. <a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601008</a>

Then, we drive the expression of FKBP-yEGFP with 3 promoters pTet07,pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry.

<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601025</a>
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601026</a>
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601027</a>

What's more, we fuse FKBP-yEGFP with HOtag3. This part can form phase separation with Frb-yEGFP-HOTag6 in the presence of rapamycin. <a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601011</a>

Then, we drive the expression of FKBP-yEGFP-HOTag6 with 2 promoters pTEF1 and PDH3. The expression of these promoters were measure by flow cytometry. <a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601037</a>
<a href="https://parts.igem.org/Part:BBa_K2601007">BBa_K2601038</a>