Difference between revisions of "Part:BBa K2680268"
 
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3G YFP flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
 
3G YFP flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
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Yellow Fluorescent Protein variant. Performs the same as <partinfo>BBa_K592101</partinfo>. From that page: "Yellow Fluorescent Protein derived from <I>Aequorea victoria</I> GFP. This sequence is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original gene was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington. [...] This part is useful as a reporter."<sup>1</sup>
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===References===
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<sup>1</sup>Lundin, E. (2011, August 21). Part:BBa_K592101. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K592101
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:21, 17 October 2018


3G YFP

3G YFP flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Yellow Fluorescent Protein variant. Performs the same as BBa_K592101. From that page: "Yellow Fluorescent Protein derived from Aequorea victoria GFP. This sequence is cloned from the pZE12-YFP plasmid used by Elowitz (see reference). The original gene was made by the The Yeast Resource Center (YRC) based at the University of Washington in Seattle, Washington. [...] This part is useful as a reporter."1

References

1Lundin, E. (2011, August 21). Part:BBa_K592101. Retrieved October 16, 2018, from https://parts.igem.org/Part:BBa_K592101

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 786