Difference between revisions of "Part:BBa K2680263"
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AraC gene from the L-arabinose operon with an added ssra tag to allow for quicker endogenous degradation in e.coli. Represses pBAD promoter in the absence of arabinose. Gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
 
AraC gene from the L-arabinose operon with an added ssra tag to allow for quicker endogenous degradation in e.coli. Represses pBAD promoter in the absence of arabinose. Gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
  
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Compare to the coding sequence for AraC, <partinfo>BBa_K1088005</partinfo>.
  
 
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Revision as of 21:09, 16 October 2018


3G AraC-ssrA

AraC gene from the L-arabinose operon with an added ssra tag to allow for quicker endogenous degradation in e.coli. Represses pBAD promoter in the absence of arabinose. Gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

Compare to the coding sequence for AraC, BBa_K1088005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 47
    Illegal BsaI.rc site found at 978