Difference between revisions of "Part:BBa K2729011"

Revision as of 18:25, 16 October 2018

DENV2 ancC-prM-E (Dengue Virus Serotype II)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis

As shown in Figure 1, the band of DENV2 prME come around 2,000 bp.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 946
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1397
Illegal AgeI site found at 1108
Illegal AgeI site found at 1450
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 349

Reference

- Membranotropic regions of the dengue virus prM protein

@@ Line 8: / Line 8: @@
 ==Characterization==
 The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.
+[[File:Tokyotech electrophoresis あはは.png|thumb|left|300px| '''Figure 1:''' '''Result of the Electrophoresis''' ]]
+<br>
+As shown in Figure 1, the band of DENV2 prME come around 2,000 bp.
+<br style="clear: both" />
 ==Sequence and Features==