Difference between revisions of "Part:BBa K2665014"
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===Usage and Biology===
 
===Usage and Biology===
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SseNHX1 is the vacuolar Na(+)/H(+) antiporter. This antiporter enables to survive high osmotic pressure by sequestering excessive cytoplasmic Na(+) into vacuole.The gene, SseNHX1 is created by DNA family shuffling;  Two homologous Na(+)/H(+) antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1) were shuffled. By reference, SseNHX1 enables higher salt tolerance and sequester Na(+) than the two protein, SeNHX1 and SsNHX1.
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This is a Na+/H+ antiporter gene, which is a chimeric gene of two homologous Na+/H+ antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1). The Na+/H+ antiporter was obtained by DNA family shuffling.<br>
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The study in 2015 has found that expression of this transporter in yeast mutant showed higher salt tolerance ability in yeast growth test than that of SsNHX1and SeNHX1.[1] It also has founded that SseNHX1 protein in yeast cells accumulated more Na+ and slightly more K+ than the proteins of SsNHX1 and SeNHX1 did.  
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<partinfo>BBa_K2665014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2665014 SequenceAndFeatures</partinfo>
  
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===Reference===
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[1]G.Wu, G.Wang et al. (2015) A chimeric vacuolar Na+/H+antiporter gene evolved by DNA family shuffling confers increased salt tolerance in yeast, Journal of Biotechnology 203 1-8
  
 
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Revision as of 14:25, 17 October 2018


TDH3 promoter->SseNHX1->6xHis->CYC terminator

SseNHX1

Usage and Biology

This is a Na+/H+ antiporter gene, which is a chimeric gene of two homologous Na+/H+ antiporters from halophytes Salicornia europaea (SeNHX1) and Suaeda salsa (SsNHX1). The Na+/H+ antiporter was obtained by DNA family shuffling.
The study in 2015 has found that expression of this transporter in yeast mutant showed higher salt tolerance ability in yeast growth test than that of SsNHX1and SeNHX1.[1] It also has founded that SseNHX1 protein in yeast cells accumulated more Na+ and slightly more K+ than the proteins of SsNHX1 and SeNHX1 did.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

[1]G.Wu, G.Wang et al. (2015) A chimeric vacuolar Na+/H+antiporter gene evolved by DNA family shuffling confers increased salt tolerance in yeast, Journal of Biotechnology 203 1-8