Difference between revisions of "Part:BBa K2817013"
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YebF is a secretion protein from E.coli. It’s used as secretion tag.We combine YebF with GFP to characterize its performance.
 
YebF is a secretion protein from E.coli. It’s used as secretion tag.We combine YebF with GFP to characterize its performance.
  
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===Usage and Biology===
 
===Usage and Biology===
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When testing the validity of the secretory tag YebF, we transformed the constructed YebF-GFP plasmid into DH5α and cultured overnight at 37 ℃. The supernatant was centrifuged at 3000 rpm for 5 min, and the supernatant was taken for fluorescence detection. The results are shown in the Figure 1. But we forgot to set up a positive control group. Due to time constraints, we have no time to repeat this experiment. But as a secretory tag used in both the literature and the iGEM team, we believe it can actually work.
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https://static.igem.org/mediawiki/2018/9/92/T--NEU_China_A--results-6.png
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Figure 1. The fluorescence of overnight bacterial suspension after centrifugation at 3000 rpm for 5 min.
  
 
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Revision as of 16:04, 16 October 2018

J23100-RBS-YebF-GFP

YebF is a secretion protein from E.coli. It’s used as secretion tag.We combine YebF with GFP to characterize its performance.

Usage and Biology

When testing the validity of the secretory tag YebF, we transformed the constructed YebF-GFP plasmid into DH5α and cultured overnight at 37 ℃. The supernatant was centrifuged at 3000 rpm for 5 min, and the supernatant was taken for fluorescence detection. The results are shown in the Figure 1. But we forgot to set up a positive control group. Due to time constraints, we have no time to repeat this experiment. But as a secretory tag used in both the literature and the iGEM team, we believe it can actually work.

T--NEU_China_A--results-6.png

Figure 1. The fluorescence of overnight bacterial suspension after centrifugation at 3000 rpm for 5 min.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1056