Difference between revisions of "Part:BBa K2686002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was codon optimized for bacterial expression in ''E. coli'' and introduced in between amino acids 43/44 in . | + | The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was codon optimized for bacterial expression in ''E. coli'' and introduced in between amino acids 43/44 in [[parts.igem.org/Part:BBa_K2686001|BBa_K2686001]] which is shifted by one amino acid downstream compared to the location in [[parts.igem.org/Part:BBa_K192000|BBa_K192000]] where it would be in between amino acids 42/43 (Moon et al., 2014). |
===Source=== | ===Source=== | ||
Revision as of 12:43, 16 October 2018
Encapsulin protein with HexaHistidine insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 492 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457
Design Notes
The HexaHistidine sequence (GGGGGGHHHHHHGGGGG) was codon optimized for bacterial expression in E. coli and introduced in between amino acids 43/44 in BBa_K2686001 which is shifted by one amino acid downstream compared to the location in BBa_K192000 where it would be in between amino acids 42/43 (Moon et al., 2014).
Source
Derived from BBa_K2686001.
References
Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801.