Difference between revisions of "Part:BBa K2764002"
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<b>Usage and biology</b> <br>
 
<b>Usage and biology</b> <br>
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<p>As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764002 utilizes the promoter BBa_J23108, which gives the biobrick a promoter strength of 0.5 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick.</p>
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<p>As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764002 utilizes the promoter BBa_J23108, which gives the biobrick a promoter strength of 0.5 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick. In this experiment, we used a bacteria transformed with a plasmid containing insert of BBa_I13401 as the control. This is because the control's plasmid has a similar size to our part's plasmid.</p>
  
 
<b>Growth curve</b><br>
 
<b>Growth curve</b><br>
 
https://static.igem.org/mediawiki/2018/d/d3/T--Hong_Kong_HKUST--BBa_002.jpeg<br>
 
https://static.igem.org/mediawiki/2018/d/d3/T--Hong_Kong_HKUST--BBa_002.jpeg<br>
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<p>In this experiment, we used a bacteria transformed with a plasmid containing insert of BBa_I13401 as the control. This is because the control's plasmid has a similar size to our part's plasmid. As shown, the growth rate of bacteria with BBa_K2764002 rises steadily, but not as rapidly as BBa_K2764001, and peaks at 0.7AU at T=8</p> <br>
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<p>As shown, the growth rate of bacteria with BBa_K2764002 rises steadily, but not as rapidly as BBa_K2764001, and peaks at 0.7AU at T=8</p> <br>
  
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<p>Compared with the growth of the control, </p>
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<p>Compared with the growth of the control, bacteria with BBa_K2764002 has lower growth throughout the 8-hour period</p>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:27, 16 October 2018


Alkane channel protein with BBa_J23108 Constitutive promoter

Usage and biology

As shown in literature, the AlkL protein may be toxic and inhibit the growth of its host. We decided to test the severity of this toxicity and compare by using Anderson promoters of varying strength. BBa_K2764002 utilizes the promoter BBa_J23108, which gives the biobrick a promoter strength of 0.5 on the Anderson promoter scale. This will be used to measure the effect of toxicity of AlkL based on the growth curve of bacteria transformed with this biobrick. In this experiment, we used a bacteria transformed with a plasmid containing insert of BBa_I13401 as the control. This is because the control's plasmid has a similar size to our part's plasmid.

Growth curve
T--Hong_Kong_HKUST--BBa_002.jpeg

As shown, the growth rate of bacteria with BBa_K2764002 rises steadily, but not as rapidly as BBa_K2764001, and peaks at 0.7AU at T=8


Compared with the growth of the control, bacteria with BBa_K2764002 has lower growth throughout the 8-hour period

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 712
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 364
    Illegal NgoMIV site found at 577
  • 1000
    COMPATIBLE WITH RFC[1000]