Difference between revisions of "Part:BBa K2596001"
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PcpcB is a constitutive promoter from cyanobacteria Synechococcus elongatus PCC 7942. | PcpcB is a constitutive promoter from cyanobacteria Synechococcus elongatus PCC 7942. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:37, 16 October 2018
PcpcB from Synechococcus elongatus PCC7942
PcpcB is a constitutive promoter from cyanobacteria Synechococcus elongatus PCC 7942.
Usage and Biology
cpcB codes for phycocyanin, a phycobiliprotein complex, and works to harvest light energy before transmitting it to the chlorophylls. The promoter is a strong promoter [4] but also demonstrates expression patterns following circadian oscillations [5]. Studies have found that Pcpc can also be used in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
PcpcB1A1 features two promoter sequences within the promoter, both of which were included in the BioBrick. For this promoter, derived from Synechococcus elongatus PCC 7942, we kept about 100 bp of upstream and downstream regions, the latter of which was cropped immediately before the ATG start codon. Studies have found that Pcpc can also be used in E. coli.