Difference between revisions of "Part:BBa K2718011"
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https://static.igem.org/mediawiki/2018/2/2a/T--Aix-Marseille--BenzylAlcohol_pathway.png
 
https://static.igem.org/mediawiki/2018/2/2a/T--Aix-Marseille--BenzylAlcohol_pathway.png
 
Figure 1 Metabolic pathway to produce benzyl alcohol
 
Figure 1 Metabolic pathway to produce benzyl alcohol
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===Production===
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We producted HmaS in ''E.coli'' DH5alpha, with 1mm IPTG at OD 0.8, 3 hours
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'
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'''Image Blue Comassis'''
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Figure 2 SDS-PAGE of HmaS production with and without induction
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 05:56, 16 October 2018


IPTG inductible promotor RBS (strong) HmaS

Usage and Biology

HmaS is used in the metabolic pathway shown in figure 1 to produce benzyl alcohol. HmaS catalyzes the oxidative decarboxylation of phenylpyruvate to produce (S)Mandelate.


T--Aix-Marseille--BenzylAlcohol_pathway.png Figure 1 Metabolic pathway to produce benzyl alcohol


Production

We producted HmaS in E.coli DH5alpha, with 1mm IPTG at OD 0.8, 3 hours ' Image Blue Comassis

Figure 2 SDS-PAGE of HmaS production with and without induction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 607
    Illegal BamHI site found at 716
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 556