Difference between revisions of "Part:BBa K2656200"
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To do so, it includes two divergently orientated BsmBI Type IIS recognition sites, whose cleavage leaves equivalent overhangs to those of the alpha 1 BsmBI digestion (Figure 1). Thus, it can be used to ligate any transcriptional unit assembled in an alpha 1 GB vector into a BioBrick compatible vector, so adapting the composite part into the BB grammar with an easy GB one-pot reaction. | To do so, it includes two divergently orientated BsmBI Type IIS recognition sites, whose cleavage leaves equivalent overhangs to those of the alpha 1 BsmBI digestion (Figure 1). Thus, it can be used to ligate any transcriptional unit assembled in an alpha 1 GB vector into a BioBrick compatible vector, so adapting the composite part into the BB grammar with an easy GB one-pot reaction. | ||
| − | This backbone has been used to adapt each our our composite parts of the [http://2018.igem.org/Team:Valencia_UPV/Part_Collection UPV iGEM 2018 Printeria Part Collection] into the | + | This backbone has been used to adapt each our our composite parts of the [http://2018.igem.org/Team:Valencia_UPV/Part_Collection UPV iGEM 2018 Printeria Part Collection] into the BioBrick standards. |
[[File:T--Valencia_UPV--BBUPV2018.png|700px|thumb|left|alt=domestication.|Figure 1. Transcriptional unit assembled in an alpha 1 GoldenBraid vector (KmR). Digestion with BsmBI Type IIS endonuclease leaves complementary overhangs (5'-GGAG-3' and 3'-CATG-5') to the pBioBrickator vector digest. ]] | [[File:T--Valencia_UPV--BBUPV2018.png|700px|thumb|left|alt=domestication.|Figure 1. Transcriptional unit assembled in an alpha 1 GoldenBraid vector (KmR). Digestion with BsmBI Type IIS endonuclease leaves complementary overhangs (5'-GGAG-3' and 3'-CATG-5') to the pBioBrickator vector digest. ]] | ||
Revision as of 21:56, 17 October 2018
pBIOBRICKATOR
BBa_K2656200 (pBioBrickator backbone) is a modification of the pSB1C3 backbone to receive transcriptional units previously assembled in a [http://2018.igem.org/Team:Valencia_UPV/Design#Level1 GB alpha 1] vector.
To do so, it includes two divergently orientated BsmBI Type IIS recognition sites, whose cleavage leaves equivalent overhangs to those of the alpha 1 BsmBI digestion (Figure 1). Thus, it can be used to ligate any transcriptional unit assembled in an alpha 1 GB vector into a BioBrick compatible vector, so adapting the composite part into the BB grammar with an easy GB one-pot reaction.
This backbone has been used to adapt each our our composite parts of the [http://2018.igem.org/Team:Valencia_UPV/Part_Collection UPV iGEM 2018 Printeria Part Collection] into the BioBrick standards.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal SpeI site found at 921
Illegal PstI site found at 935 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal NheI site found at 44
Illegal NheI site found at 67
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal NotI site found at 11
Illegal NotI site found at 928 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5
Illegal XhoI site found at 1952
Illegal XhoI site found at 2844 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 5
Illegal suffix found at 921 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 5
Plasmid lacks a suffix.
Illegal XbaI site found at 20
Illegal SpeI site found at 921
Illegal PstI site found at 935
Illegal AgeI site found at 653
Illegal AgeI site found at 765 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.