Difference between revisions of "Part:BBa K2559007"

Latest revision as of 09:20, 16 October 2018

Promoter of Heat Shock Proteins (HSPs) which consists of GroEL and GroES

Usage and Biology

The DNA sequence of Heat Shock Proteins (HSPs) in Synechococcus sp. PCC7942 had been figured out, which consists of GroEL and GroES.[1]It allows us to use the culture temperature to regulate the expression of downstream genes.

The promoter of HSPs is a strong promoter.About 2.3 kb transcript of groESL1 operon increased 30-fold within 30 min upon heat shock. It allows us to regulate the expression of target genes by changing the culture temperature, and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.[1]

In our project，we have constructed the inducible expressing shuttle plasmid pgFQ and inserted bacterial cellulose into the homologous arm slr0168 (as shown in the figure below). We transformed the Synechocystis and measure the bacterial cellulose content of the transformants.

Figure 1:Bacteria cellulose induced expression cassette pgFQ

Reference:

[1]Biochimica et Biophysica Acta, Cloning, characterization and functional analysis of groESL operon from thermophilic cyanobacterium Synechococcus vulcanus, (1998)1343(2):335-48

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
-The DNA sequence of Heat Shock Proteins (HSPs) in Synechococcus sp. PCC7942 has been figured out, which consists of GroEL and GroES.[1]It allows us to use the culture temperature to regulate the expression of downstream genes
+The DNA sequence of Heat Shock Proteins (HSPs) in ''Synechococcus sp. PCC7942 ''had been figured out, which consists of GroEL and GroES.[1]It allows us to use the culture temperature to regulate the expression of downstream genes.
-The promoter of HSPs is a strong promoter, the amount of the bicistronic, 2.3 kb transcript of groESL1 operon increased 30-fold within 30 min upon heat shock. It allows us to regulate the expression of target genes by changing the culture temperature, and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.[1]
-In our project，we  have constructed the inducible expression shuttle plasmid pgFQ and inserted bacterial cellulose into the homologous arm slr0168 (as shown in the figure below). We will transform the Synechocystis and measure the bacterial cellulose content of the transformants.
+The promoter of HSPs is a strong promoter.About 2.3 kb transcript of groESL1 operon increased 30-fold within 30 min upon heat shock. It allows us to regulate the expression of target genes by changing the culture temperature, and avoid the risk of losing transformed algae strains for toxic effect caused by target genes.[1]
+In our project，we  have constructed the inducible expressing shuttle plasmid pgFQ and inserted bacterial cellulose into the homologous arm'' slr0168'' (as shown in the figure below). We transformed the ''Synechocystis'' and measure the bacterial cellulose content of the transformants.
 [[File:Scau-china-2018-13.png|800px|thumb|left|	Figure 1:Bacteria cellulose induced expression cassette pgFQ]]