Difference between revisions of "Part:BBa K2842669"
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[[File:T--UCL--IP_IPTG_Variations.png|400px|thumb|right|
 
[[File:T--UCL--IP_IPTG_Variations.png|400px|thumb|right|
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<center>'''Figure 1: BsaI digestions'''</center>
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<center>'''Figure 1: Expression of IP at varying IPTG concentrations'''</center>
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<p>
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Initial experiments suggested that 400 uM of IPTG was optimal for expression(data not shown). A plate reader was used to examine the region of IPTG concentrations around 400 uM
 
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Revision as of 18:15, 15 October 2018


mScarlet reporter with TerL-C intein on the N terminus

Figure 1: Expression of IP at varying IPTG concentrations

Initial experiments suggested that 400 uM of IPTG was optimal for expression(data not shown). A plate reader was used to examine the region of IPTG concentrations around 400 uM

Figure 1: BsaI digestions
<p> (1) BsaI digested Intein Passenger BBa_K2842669
(2) BsaI digested RFP inteins BBa_K2842680
(3) BsaI digested GFP inteins BBa_K2842690
*edited to show relevant bands

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1224
    Illegal BsaI.rc site found at 28
    Illegal SapI site found at 1130
    Illegal SapI.rc site found at 419