Difference between revisions of "Part:BBa K2680280"
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3G TlpA39 is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
 
3G TlpA39 is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
  
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3G TlpA39 is a a temperature sensitive repressor mtuated from Tlpa. Tlpa is a " tunable, orthogonal, temperature-dependent transcriptional repressor providing switch-like control of bacterial gene expression at thresholds spanning the biomedically relevant range of 32-46 °C" <sup>1</sup> Tlpa39 is shifted to have depression occur at a higher temperature.  
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3G TlpA39 is a a temperature sensitive repressor mtuated from Tlpa. Tlpa is a " tunable, orthogonal, temperature-dependent transcriptional repressor providing switch-like control of bacterial gene expression at thresholds spanning the biomedically relevant range of 32-46 °C" <sup>1</sup> Tlpa39 is mutated so that its derepression occurs at a higher temperature, 39 C
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===References===
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1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.
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Revision as of 15:43, 16 October 2018


3G TlpA39

3G TlpA39 is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

3G TlpA39 is a a temperature sensitive repressor mtuated from Tlpa. Tlpa is a " tunable, orthogonal, temperature-dependent transcriptional repressor providing switch-like control of bacterial gene expression at thresholds spanning the biomedically relevant range of 32-46 °C" 1 Tlpa39 is mutated so that its derepression occurs at a higher temperature, 39 C 

===References===

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1170