Difference between revisions of "Part:BBa K2680281"
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3G LsRK is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. | 3G LsRK is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page]. | ||
Revision as of 16:02, 15 October 2018
3G LsRK
3G LsRK is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
LsrK is a kinase involved in a LuxS/AI-2 signaling pathway in Salmonella Typhimurium. This kinase catalyzes the phosphorylation of autoinducer-2 (AI-2) to phospho-AI-2. AI-2 acts as a "universal signal" for interspecies communication for quorum sensing. 1
References
1 Wang, H., & I. (2015, September 6). Part:BBa_K1666004. Retrieved from https://parts.igem.org/Part:BBa_K1666004
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 109
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 1647