Difference between revisions of "Part:BBa K2796028"
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metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of | metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of | ||
these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)</li> | these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)</li> | ||
| + | Here is the final device that we designed for this project. | ||
[[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]] | [[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]] | ||
| + | More details you can visit in our wiki! | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:03, 16 October 2018
Exosome booster
- Exosome booster refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified STEAP3 (involved in exosomebiogenesis), syndecan-4(SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 192
Illegal BglII site found at 1777 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2657
Illegal AgeI site found at 3277 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1367
Here is the final device that we designed for this project.
More details you can visit in our wiki! Sequence and Features
Assembly Compatibility: