Difference between revisions of "Part:BBa K2656019"

Revision as of 14:32, 15 October 2018

LuxI Coding Sequence

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Gate] assembly methods. It was codon optimized for E. coli.

LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.

It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161  BBa_C0161] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 GoldenBraid 3.0] assemply methods. It was codon optimized for ''E. coli''.
+Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161  BBa_C0161] adapted to the Golden Braid grammar and so used with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Gate] assembly methods. It was codon optimized for ''E. coli''.
+LuxI coding sequence codifies for the 3OC6HSL (AHL) synthetase, so two signaling molecules of AHL interacts with two luxR proteins creating a complex which binds to the pLux promoter.
 It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a one-step reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].