Difference between revisions of "Part:BBa K2721010"
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<partinfo>BBa_K2721010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2721010 SequenceAndFeatures</partinfo> | ||
| − | + | ==Usage and Biology== | |
CsgA is the monomer of curli, which is an important component of excellular matrix of Enterobacteriaceae. Curli fibers are involved in adhesion to surfaces, cell aggregation, and biofilm formation, they are also used for studies of amyloid fiber formation in human diseases, such as Alzheimer’s, Huntington’s, and prion diseases. | CsgA is the monomer of curli, which is an important component of excellular matrix of Enterobacteriaceae. Curli fibers are involved in adhesion to surfaces, cell aggregation, and biofilm formation, they are also used for studies of amyloid fiber formation in human diseases, such as Alzheimer’s, Huntington’s, and prion diseases. | ||
Revision as of 02:55, 16 October 2018
the gene of csgA
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
CsgA is the monomer of curli, which is an important component of excellular matrix of Enterobacteriaceae. Curli fibers are involved in adhesion to surfaces, cell aggregation, and biofilm formation, they are also used for studies of amyloid fiber formation in human diseases, such as Alzheimer’s, Huntington’s, and prion diseases.
As an important component of excellular matrix and biofilms, modified csgA provides us many potentials to remould biofilm into a brand-new functional biology matrix. We can tack many fancy characteristics to biofilm and modified bacteria. Team ZJU-CHINA 2018 made use of the design of cell-surface display and modified the csgA protein by fusing spytag to the N-terminal, making it a dorable platform to form enzyme complex and increasd the electrical conductivity because of the histag in the C-terminal.Actually, the cell-surface display has many potential applications, including biodegradation,live vaccine development, peptide library screening, bioconversion using whole cell biocatalyst and bioadsorption.
As the excellular matrix of bacteria, the curli can also interact with intracellular reactions, and function as a detector of environment or messenger between extracellular and intracellular. For example, the modified curli can be used as a membrane-based bisensor for pathogen, heavy metal and other environment pollutants.