Difference between revisions of "Part:BBa K2559002"

Revision as of 09:24, 15 October 2018

--NOTOC-- Bacterial cellulose synthase X(BglX)

The BBa_K2559001 coding a β-glucosidase, which is occasionally encoded in the bcs operons.

Usage and Biology

BGLX is a β-glucosidase which participates the cyanoamino acid metabolism (ko00460), starch and sucrose metabolism (ko00500), phenylpropanoid biosynthesis (ko00940), metabolic pathways (ko01100), biosynthesis of secondary metabolites (ko01110). The enzyme is expected to participate in hydrolysis of bacterial cellulose, instead of synthesis. Its role in cellulose biosynthesis remains to be further explored.

The pathways involve bcsH（entry 3.2.1.21） in Starch and sucrose metabolism(ko00500)

We transfered bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria. Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement Cyanobacteria glucose content measurement (3 repeats). The same of quality of transferred cyanobacteria with bcsZH-ABCD-bglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable.

The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group and control group.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 516
Illegal XhoI site found at 1206
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2099
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 339

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The products of bglX is a β-glucosidase . It participate the Cyanoamino acid metabolism(ko00460),Starch and sucrose metabolism(ko00500),Phenylpropanoid biosynthesis(ko00940),Metabolic pathways(ko01100),Biosynthesis of secondary metabolites(ko01110)
+BGLX is a β-glucosidase which participates the cyanoamino acid metabolism (ko00460), starch and sucrose metabolism (ko00500), phenylpropanoid biosynthesis (ko00940), metabolic pathways (ko01100), biosynthesis of secondary metabolites (ko01110).
-Such enzymes could be expected to participate in bacterial cellulose hydrolysis,  rather than synthesis ,of β-D-glucans, and  roles in cellulose biosynthesis have long remained obscure.
+The enzyme is expected to participate in hydrolysis of bacterial cellulose, instead of synthesis. Its role in cellulose biosynthesis remains to be further explored.
 [[File:Scau-china-2018-10.png|800px|thumb|center|The pathways involve bcsH（entry 3.2.1.21） in Starch and sucrose metabolism(ko00500)]]
 We transfered bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria.