Difference between revisions of "Part:BBa K2656109"
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030]  medium strength ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030]  medium strength ribosome biding site in its Golden Braid compatible version <html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656014 BBa_K2656014]: the [https://parts.igem.org/Part:BBa_E1010 BBa_E1010] mRFP1 sequence in its Golden Braid standardized version <html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656014 BBa_K2656014]: the [https://parts.igem.org/Part:BBa_E1010 BBa_E1010] mRFP1 sequence in its Golden Braid standardized version <html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
  
 
<p> Thus, in this transcriptional unit mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS.</p>
 
<p> Thus, in this transcriptional unit mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS.</p>
 
</html>
 
</html>
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The characterization of the mRFP1 protein (and by extension of all the other part that codify for the mRFP1) was performed with this transcriptional unit. In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we assembled with it,  the emission and excitation spectra were obtained using [http://2018.igem.org/Team:Valencia_UPV/Experiments#imReporter this protocol].
  
  

Revision as of 23:13, 14 October 2018


mRFP1 transcriptional unit 1

Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

  • BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
  • BBa_K2656009: the B0030 medium strength ribosome biding site in its Golden Braid compatible version
  • BBa_K2656014: the BBa_E1010 mRFP1 sequence in its Golden Braid standardized version
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

    • Thus, in this transcriptional unit mRFP1 sequence is assembled with a very low strength promoter and relative strong RBS.

    The characterization of the mRFP1 protein (and by extension of all the other part that codify for the mRFP1) was performed with this transcriptional unit. In order to carry out a correct characterization of the protein and to be able to use it to make measurements of the different transcriptional units that we assembled with it, the emission and excitation spectra were obtained using [http://2018.igem.org/Team:Valencia_UPV/Experiments#imReporter this protocol].


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 11
      Illegal NheI site found at 34
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 620
      Illegal AgeI site found at 732
    • 1000
      COMPATIBLE WITH RFC[1000]