Difference between revisions of "Part:BBa K2582001"
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This biobrick is a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2582000 miniSpinach]-based RNA Aptamer Probe (RAP). It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.
 
This biobrick is a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2582000 miniSpinach]-based RNA Aptamer Probe (RAP). It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.
  
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[[File:BBa K2582001-illustration.png|600px|thumb|left|Illustration of the design]]
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<p style="clear:left"></p>
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===Usage and Biology===
 
===Usage and Biology===
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The full miniSpinach allows the docking of fluorogen 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) and increases its quantum yields by hundreds of folds. By truncating the critical P1 arm and attaching it to a 22-bp RNA-specific probe, the RNA Aptamer Probe can bind to a RNA sequence and enhances the fluorecence level of the docking DFHBI subsequently. In our work, this probe is demonstrated to be functional in vitro, targeting influenza RNA fragments. It also has the potential for live-cell reporting of mRNA level (Ong. et. al. 2017.).
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The probe can be used for on-site, rapid and sequence-specific influenza detection. Its target neuraminidase gene encodes for one of the influenza viral protein that determines the subtype of the virus, which is a important factor for epidemic monitoring and some treatment schemes.
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===Characterization===
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<h4><i>Time</i></h4>
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<h4><i>Temperature</i></h4>
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<h4><i>Limit of Detection</i></h4>
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<h4><i>Salt Concentration</i></h4>
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<h4><i>Specificity</i></h4>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2582001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2582001 SequenceAndFeatures</partinfo>

Revision as of 03:22, 15 October 2018


RNA Aptamer Probe for N9 (545-566)

This biobrick is a miniSpinach-based RNA Aptamer Probe (RAP). It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.

Illustration of the design

Usage and Biology

The full miniSpinach allows the docking of fluorogen 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) and increases its quantum yields by hundreds of folds. By truncating the critical P1 arm and attaching it to a 22-bp RNA-specific probe, the RNA Aptamer Probe can bind to a RNA sequence and enhances the fluorecence level of the docking DFHBI subsequently. In our work, this probe is demonstrated to be functional in vitro, targeting influenza RNA fragments. It also has the potential for live-cell reporting of mRNA level (Ong. et. al. 2017.).

The probe can be used for on-site, rapid and sequence-specific influenza detection. Its target neuraminidase gene encodes for one of the influenza viral protein that determines the subtype of the virus, which is a important factor for epidemic monitoring and some treatment schemes.

Characterization

Time

Temperature

Limit of Detection

Salt Concentration

Specificity

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]