Difference between revisions of "Part:BBa K2559001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
It is encoded exclu-sively in the Komagataeibacter/Gluconacetobacter lineage and is required for the BCS activity in K. xylinus and Komagataeibacter hansenii It has been shown to interact with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons. in the periplasm. This unique organization might account for the extremely high activity of the K. xylinus BCS. | It is encoded exclu-sively in the Komagataeibacter/Gluconacetobacter lineage and is required for the BCS activity in K. xylinus and Komagataeibacter hansenii It has been shown to interact with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons. in the periplasm. This unique organization might account for the extremely high activity of the K. xylinus BCS. | ||
| − | [[File:Scau-china-2018-6.png|800px|thumb|center| Figure 1 The double digestion of biobrick by EcoRI and pstI]] | + | [[File:Scau-china-2018-6.png|800px|thumb|center|Figure 1 The double digestion of biobrick by EcoRI and pstI]] |
Transgenic cyanobacteria with BcsZH-ABCD-BglX genes and the cellulose measurement | Transgenic cyanobacteria with BcsZH-ABCD-BglX genes and the cellulose measurement | ||
Cyanobacteria glucose content measurement (3 repeats). The same of quality of transferred cyanobacteria with BcsZH-ABCD-BglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable. | Cyanobacteria glucose content measurement (3 repeats). The same of quality of transferred cyanobacteria with BcsZH-ABCD-BglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable. | ||
Revision as of 16:01, 14 October 2018
Bacterial cellulose synthase H (BcsH)
The BBa_K2559001 is coding a cellulose complement protein A (ccpA) . https://www.sciencedirect.com/science/article/pii/S0966842X15001201#tbl0005
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 746
Illegal AgeI site found at 907 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 351