Difference between revisions of "Part:BBa K2841500"
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[[Image:https://static.igem.org/mediawiki/parts/4/4c/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph.png|400px|right|oof]]
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[[Image:T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph.png|1250px|center|IPTG Induction]]
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[[Image:T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-OD600.png|1250px|center|IPTG OD600]]
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[[Image:T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-Raw-Flourescence.png|1250px|center|IPTG Induction Fluorescence]]
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Revision as of 13:52, 14 October 2018


Legionella Sensing LacI Repressing Alpha Toxicity Experimental Device

GFP is expressed with the gRNA to test toxicity


IPTG Induction
IPTG OD600
IPTG Induction Fluorescence




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 856