Difference between revisions of "Part:BBa K2656100"

Revision as of 21:30, 14 October 2018

sfGFP transcriptional unit 1

Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design#Level1 Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

BBa_K2656004: the J23106 constitutive promoter in its Golden Braid compatible version
BBa_K2656008: the J61100 very weak ribosome biding site in its Golden Braid compatible version
BBa_K2656013: the BBa_I746916 sfGFP sequence in its Golden Braid standardized version
Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version

This transcriptional unit has been used to characterize the RBS basic part BBa_K2656008. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and rationale choose its optimized values.

Figure 1. RBS expression experiment with K2656008, K2656009 and K2656012 basic pieces

Figure 2. RBS expression experiment with K2656008 and K2656012 basic pieces

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 77

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+This transcriptional unit has been used to characterize the RBS basic part [https://parts.igem.org/Part:BBa_K2656008 BBa_K2656008]. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and rationale choose its optimized values.
+[[File:T--Valencia_UPV--optimization_exp2_RBS_all_graph_expUPV2018.png|900px|thumb|none|alt=RBS experiment.|Figure 1. RBS expression experiment with K2656008, K2656009 and K2656012 basic pieces]]
+[[File:T--Valencia_UPV--optimization_exp2_RBS_2obj_graph_expUPV2018.png|900px|thumb|none|alt=RBS experiment.|Figure 2. RBS expression experiment with K2656008 and K2656012 basic pieces]]