Difference between revisions of "Part:BBa K2637038"
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<partinfo>BBa_K2637038 short</partinfo> | <partinfo>BBa_K2637038 short</partinfo> | ||
| − | We constructed this cassette which played a prominent role in our measurement process | + | We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal1 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on. |
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Revision as of 05:09, 14 October 2018
Gal1 promoter+NanoLuc+ADH1 terminator
We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal1 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]